Vgamma9Vdelta2 T cells recovered from eyes of patients with Behçet's disease recognize non-peptide prenyl pyrophosphate antigens

J Neuroimmunol. 2002 Sep;130(1-2):46-54. doi: 10.1016/s0165-5728(02)00208-4.


The phenotype and antigen-specificity of T cells expanded by mitogenic stimulation from intra-ocular fluid (IOF) samples of affected eyes of six Behçet's disease (BD) patients, and seven patients with other uveitis entities, were determined. High numbers of gammadelta T cells, predominantly Vgamma9Vdelta2 T cells, were only detected in the IOF-derived TCL of three BD patients. Whereas no TCL responded to heat shock protein (HSP) 65 kDa, reactivity to isopentyl pyrophosphate (IPP) and related non-peptide prenyl pyrophosphates (PPP) was restricted to the gammadelta T cell containing TCL. Upon IPP stimulation, these TCL secreted IFN-gamma but no IL-4. By single-cell analysis of intracellular IFN-gamma production and CD69 expression the IOF-derived IPP-specific T cells were identified as CD4(-)CD8(-) gammadelta T cells. The data presented suggest the infiltration of PPP-specific Vgamma9Vdelta2 Th1-like cells into the eye of BD patients with uveitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antigen Presentation / immunology
  • Antigens, CD / immunology
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Aqueous Humor / immunology
  • Behcet Syndrome / immunology*
  • Behcet Syndrome / pathology
  • Behcet Syndrome / physiopathology
  • CD3 Complex / immunology
  • CD4-CD8 Ratio
  • Chemotaxis, Leukocyte / immunology
  • Child
  • Cytokines / immunology
  • Cytokines / metabolism
  • Diphosphates / immunology*
  • Epitopes / immunology*
  • Eye / immunology*
  • Eye / pathology
  • Eye / physiopathology
  • Female
  • Flow Cytometry
  • Humans
  • Interferon-gamma / immunology
  • Lectins, C-Type
  • Male
  • Middle Aged
  • Protein Prenylation / immunology*
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • T-Lymphocytes / immunology*


  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • CD69 antigen
  • Cytokines
  • Diphosphates
  • Epitopes
  • Lectins, C-Type
  • Receptors, Antigen, T-Cell, gamma-delta
  • Interferon-gamma