Novel orphanin FQ/nociceptin transcripts are expressed in human immune cells

J Neuroimmunol. 2002 Sep;130(1-2):100-8. doi: 10.1016/s0165-5728(02)00217-5.

Abstract

The opioid-like receptor (NOP) is widely expressed throughout the human immune system. Here, we report that human peripheral blood lymphocytes (PBLs) express transcripts encoding the NOP receptor agonist, orphanin FQ/nociceptin (OFQ/N). OFQ/N transcripts in resting PBLs were restricted to CD19+B cells and contained a novel 5' exon (ImEx2b), replacing exons 1 and 2 found in neuronal transcripts. Translation of ImEx2b-containing transcripts resulted in truncated OFQ/N precursors lacking a classical signal peptide. Mitogen activation of PBLs dramatically up-regulated neuronal-like transcripts, predominantly in CD3+T cells. Overall, this suggests different promoters direct specific OFQ/N transcript expression in immune cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing / genetics*
  • Amino Acid Sequence / genetics
  • Antigens, CD19 / genetics
  • Antigens, CD19 / immunology
  • B-Lymphocytes / immunology
  • Base Sequence / genetics
  • Cell Culture Techniques
  • Gene Expression / genetics
  • Gene Expression Regulation / immunology
  • Humans
  • Lymphocytes / cytology
  • Lymphocytes / immunology*
  • Molecular Sequence Data
  • Opioid Peptides / genetics*
  • Promoter Regions, Genetic / genetics
  • RNA Splice Sites / genetics
  • RNA, Messenger / genetics*
  • Receptors, Opioid / genetics
  • Spleen / cytology
  • Spleen / immunology
  • Transcriptional Activation / genetics

Substances

  • Antigens, CD19
  • Opioid Peptides
  • RNA Splice Sites
  • RNA, Messenger
  • Receptors, Opioid
  • nociceptin
  • nociceptin receptor