Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E

Biol Pharm Bull. 2002 Sep;25(9):1133-6. doi: 10.1248/bpb.25.1133.


The aim of this study was to determine the effects of Hippophae rhamnoides L. extract (HRe-1) and also vitamin E as a positive control on nicotine-induced oxidative stress in rat blood, specifically alterations in erythrocyte malondialdehyde (MDA) level, activities of some erythrocyte antioxidant enzymes, and plasma vitamin E and A levels. The groups were: nicotine (0.5 mg/kg/d, intraperitoneal, i.p.); nicotine+vitamin E (75 mg/kg/d, intragastric, i.g.); nicotine+HRe-1 (1 ml/kg/d, i.g.); and control group (receiving only vehicles). There were 8 rats per group and the supplementation period was 3 weeks. Nicotine-induced increase in erythrocyte MDA level was prevented by both HRe-1 and vitamin E. Nicotine-induced decrease in erythrocyte superoxide dismutase (SOD) activity was prevented by HRe-1, but not vitamin E. HRe-1 increased the erythrocyte glutathione peroxidase (GSH-Px) activity compared with nicotine and the vitamin E groups. Catalase activity was not affected. Vitamin E supplementation increased plasma vitamin E level. Plasma vitamin A level was higher in both vitamin E and HRe-1 supplemented groups compared with nicotine and control groups. The results suggest that HRe-1 extract can be used as a dietary supplement, especially by people who smoke, in order to prevent nicotine-induced oxidative stress.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fruit
  • Glutathione Peroxidase / blood
  • Hippophae*
  • Malondialdehyde / blood
  • Nicotine / pharmacology*
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / blood
  • Vitamin E / pharmacology*


  • Plant Extracts
  • Vitamin E
  • Malondialdehyde
  • Nicotine
  • Glutathione Peroxidase
  • Superoxide Dismutase