AppA is a blue light photoreceptor that antirepresses photosynthesis gene expression in Rhodobacter sphaeroides

Cell. 2002 Sep 6;110(5):613-23. doi: 10.1016/s0092-8674(02)00876-0.

Abstract

Photosynthetic bacteria regulate photosystem synthesis in response to alterations in oxygen tension and light intensity. In this study we show that the PpsR repressor from Rhodobacter sphaeroides binds to DNA in a redox-dependent manner through the formation/breakage of an intramolecular disulfide bond. We also demonstrate that PpsR is antagonized by the flavin-containing antirepressor, AppA, that is capable of breaking the disulfide bond in oxidized PpsR as well as forming a stable AppA-PpsR(2) antirepressor-repressor complex. Blue light excitation of AppA induces a photocycle that is characterized by a long-lived red-shifted absorbance of the flavin. Light-excited AppA was found to be incapable of forming the AppA-PpsR(2) antirepressor complex. These results establish AppA as a transcription factor that controls both redox and blue light repression of photosystem gene expression by mediating DNA binding activity of PpsR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • DNA, Bacterial / metabolism
  • DNA-Binding Proteins / metabolism
  • Disulfides / metabolism
  • Flavins / metabolism
  • Flavoproteins / physiology*
  • Gene Expression Regulation, Bacterial
  • Oxidation-Reduction
  • Photoreceptors, Microbial*
  • Photosynthesis*
  • Repressor Proteins / metabolism
  • Rhodobacter sphaeroides / genetics*
  • Transcription Factors

Substances

  • AppA protein, Rhodobacter sphaeroides
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Disulfides
  • Flavins
  • Flavoproteins
  • Photoreceptors, Microbial
  • Repressor Proteins
  • Transcription Factors