Cloning and characterization of the 5'-flanking region of the rat neuron-specific Class III beta-tubulin gene

Gene. 2002 Jul 10;294(1-2):269-77. doi: 10.1016/s0378-1119(02)00801-6.


The promoter regions of several neuron-specific structural proteins (e.g. neurofilaments, peripherin, Talpha1-tubulin) have revealed potential regulatory elements that could contribute to the choice of a neuronal phenotype during development. We initiated study of the 5'-flanking region of the rat Class III neuron-specific beta-tubulin gene (betaIII-tubulin) because this gene is expressed at the time of terminal mitosis only in neurons and thus its promoter should be an excellent tool for studying neuron-specific gene expression during the transition from proliferative progenitor cell to early neuronal differentiation. We identified the minimal promoter region needed to drive expression of the betaIII-tubulin gene. This minimal region contains multiple putative binding sites for the transcription factors SP1 and AP2, as well as a central nervous system enhancer regulatory element and an E-box. A primer extension analysis identifies a single transcription start site. We highlight several putative regulatory elements that may modulate the expression of the betaIII-tubulin gene in a stage- and tissue-specific manner. In addition, we show that the first 490 bp of the promoter are sufficient to regulate betaIII-tubulin gene expression during neuronal differentiation of PCC7 cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 5' Flanking Region / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / genetics
  • DNA / isolation & purification
  • Molecular Sequence Data
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Regulatory Sequences, Nucleic Acid / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Initiation Site
  • Tubulin / genetics*
  • Tumor Cells, Cultured


  • Recombinant Fusion Proteins
  • Tubulin
  • DNA
  • Chloramphenicol O-Acetyltransferase

Associated data

  • GENBANK/AF458477