UVB-irradiated dendritic cells induce nonproliferating, regulatory type T cells

Skin Pharmacol Appl Skin Physiol. Sep-Oct 2002;15(5):330-4. doi: 10.1159/000064537.

Abstract

It is well established that low-dose UVB radiation inhibits the antigen-presenting cell (APC) function of murine Langerhans cells in vivo and converts them from immunogenic to tolerogenic APC. Recently, we have shown that UVB-irradiated murine bone marrow-derived dendritic cells (UVB-DC) suppressed proliferation of naive and primed T cells, but tolerized primed T cells only. To examine the underlying mechanism for these differences, naive OVA(323-339)-peptide-specific, TCR-transgenic T cells from DO11.10 mice were analyzed following coculture with unirradiated DC or UVB-DC. First, we found that UVB-DC inhibit OVA-specific T cell proliferation UVB dose and antigen dose dependently. Analysis of T cells cocultured with both, unirradiated and UVB-DC, revealed an activated T cell phenotype with increased expression of CD25 and CD69 by FACS. Supernatants harvested from cocultures with UVB-DC showed reduced levels of IFN-gamma, IL-2 and IL-4, but not TGF-beta, compared with unirradiated DC as determined by ELISA. Furthermore, these T cells did not proliferate upon restimulation. Interestingly, addition of these nonproliferating T cells to cocultures of naive T cells and freshly prepared unirradiated DC inhibited T cell proliferation depending on the number of added nonproliferating T cells. Also, in supernatants, increased levels of TGF-beta were found. Therefore, our data indicate that UVB-DC propagate T cells with a regulatory function. Since regulatory T cells are characterized by enhanced TGF-beta secretion and increased CTLA-4 expression, we currently investigate the role of CTLA-4 phenotypically and functionally. In conclusion, we have shown that UVB-DC inhibit proliferation of naive OVA-specific T cells. These T cells, exhibiting an activated phenotype and increased TGF-beta production, suppress proliferation of naive T cells cocultured with unirradiated DC. These results suggest that UVB-DC induce nonproliferating, regulatory type T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Division / physiology
  • Cell Division / radiation effects
  • Dendritic Cells / cytology
  • Dendritic Cells / metabolism
  • Dendritic Cells / radiation effects*
  • Humans
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocyte Subsets / radiation effects*
  • Ultraviolet Rays* / adverse effects