Abstract
Recombinant beta-1,4-galactosyltranferase (beta 1,4-GalT) and alpha-2,6-sialytransferase (alpha 2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu-5Ac alpha(2-->6)Gal beta(1-->4)GlcNAc beta-O-(CH2)6-NH2, on a water-soluble primer having GlcNAc residues through a alpha-chymotrypsin-sensitive linker.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chymotrypsin / chemistry*
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Chymotrypsin / metabolism
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Glycoconjugates / chemical synthesis*
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Glycosylation
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N-Acetyllactosamine Synthase / chemistry*
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N-Acetyllactosamine Synthase / metabolism
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Oligosaccharides / chemical synthesis*
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Oligosaccharides / chemistry
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Polymers
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Recombinant Proteins / chemistry*
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Recombinant Proteins / metabolism
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Sepharose / chemistry
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Sialyltransferases / chemistry
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Sialyltransferases / metabolism
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Solubility
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Uridine Diphosphate Galactose / metabolism
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Water
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beta-D-Galactoside alpha 2-6-Sialyltransferase
Substances
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Glycoconjugates
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Oligosaccharides
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Polymers
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Recombinant Proteins
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Water
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Uridine Diphosphate Galactose
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Sepharose
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N-Acetyllactosamine Synthase
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Sialyltransferases
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Chymotrypsin
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beta-D-Galactoside alpha 2-6-Sialyltransferase