Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports

Chem Commun (Camb). 2001 Oct 7:(19):1944-5. doi: 10.1039/b104896c.

Abstract

Recombinant beta-1,4-galactosyltranferase (beta 1,4-GalT) and alpha-2,6-sialytransferase (alpha 2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu-5Ac alpha(2-->6)Gal beta(1-->4)GlcNAc beta-O-(CH2)6-NH2, on a water-soluble primer having GlcNAc residues through a alpha-chymotrypsin-sensitive linker.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chymotrypsin / chemistry*
  • Chymotrypsin / metabolism
  • Glycoconjugates / chemical synthesis*
  • Glycosylation
  • N-Acetyllactosamine Synthase / chemistry*
  • N-Acetyllactosamine Synthase / metabolism
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry
  • Polymers
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / metabolism
  • Sepharose / chemistry
  • Sialyltransferases / chemistry
  • Sialyltransferases / metabolism
  • Solubility
  • Uridine Diphosphate Galactose / metabolism
  • Water
  • beta-D-Galactoside alpha 2-6-Sialyltransferase

Substances

  • Glycoconjugates
  • Oligosaccharides
  • Polymers
  • Recombinant Proteins
  • Water
  • Uridine Diphosphate Galactose
  • Sepharose
  • N-Acetyllactosamine Synthase
  • Sialyltransferases
  • Chymotrypsin
  • beta-D-Galactoside alpha 2-6-Sialyltransferase