An Aeromonas salmonicida type IV pilin is required for virulence in rainbow trout Oncorhynchus mykiss

Dis Aquat Organ. 2002 Aug 15;51(1):13-25. doi: 10.3354/dao051013.

Abstract

Aeromonas salmonicida expresses a large number of proven and suspected virulence factors including bacterial surface proteins, extracellular degradative enzymes, and toxins. We report the isolation and characterization of a 4-gene cluster, tapABCD, from virulent A. salmonicida A450 that encodes proteins homologous to components required for type IV pilus biogenesis. One gene, tapA, encodes a protein with high homology to type IV pilus subunit proteins from many gram-negative bacterial pathogens, including Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio vulnificus. A survey of A. salmonicida isolates from a variety of sources shows that the tapA gene is as ubiquitous in this species as it is in other members of the Aeromonads. Immunoblotting experiments demonstrate that it is expressed in vitro and is antigenically conserved among the A. salmonicida strains tested. A mutant A. salmonicida strain defective in expression of TapA was constructed by allelic exchange and found to be slightly less pathogenic for juvenile Oncorhynchus mykiss (rainbow trout) than wild type when delivered by intraperitoneal injection. In addition, fish initially challenged with a high dose of wild type were slightly more resistant to rechallenge with wild type than those initially challenged with the tapA mutant strain, suggesting that presence of TapA contributes to immunity. Two of the other three genes identified, tapB and tapC, encode proteins with homology to factors known to be required for type IV pilus assembly in P. aeruginosa, but in an as yet unidentified manner. TapB is a member of the ABC-transporter family of proteins that contain characteristic nucleotide-binding regions, and which may provide energy for type IV pilus assembly through the hydrolysis of ATP. TapC homologs are integral cytoplasmic membrane proteins that may play a role in pilus anchoring or initiation of assembly. The fourth gene, tapD, encodes a product that shares homology with a family of proteins with a known biochemical function, namely, the type IV prepilin leader peptidases. These bifunctional enzymes proteolytically cleave the leader peptide from the pilin precursor (prepilin) and then N-methylate the newly exposed N-terminal amino acid prior to assembly of the subunits into the pilus structure. We demonstrate that A. salmonicida TapD is able to restore type IV pilus assembly and type II secretion in a P. aeruginosa strain carrying a mutation in its type IV peptidase gene, suggesting that it plays the same role in A. salmonicida.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aeromonas / genetics*
  • Aeromonas / pathogenicity
  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins*
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Endopeptidases / genetics
  • Fimbriae Proteins / genetics
  • Fimbriae Proteins / metabolism*
  • Fimbriae, Bacterial / genetics*
  • Fish Diseases / microbiology*
  • Gram-Negative Bacterial Infections / genetics
  • Gram-Negative Bacterial Infections / veterinary*
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics
  • Methyltransferases / genetics
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics
  • Multigene Family
  • Oncorhynchus mykiss*
  • Peptide Hydrolases / genetics
  • Peptide Hydrolases / metabolism
  • Sequence Alignment
  • Virulence / genetics

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA-Binding Proteins
  • Heat-Shock Proteins
  • Multienzyme Complexes
  • TapA protein, Pseudomonas
  • TapB protein, Pseudomonas fragi
  • Fimbriae Proteins
  • Methyltransferases
  • Endopeptidases
  • Peptide Hydrolases
  • TapD protein, Aeromonas