A new wc-1 mutant of Neurospora crassa shows unique light sensitivity in the circadian conidiation rhythm

Mol Genet Genomics. 2002 Sep;268(1):56-61. doi: 10.1007/s00438-002-0722-1. Epub 2002 Jul 13.

Abstract

A new clock mutant ( rhy-2) was isolated by DNA insertion mutagenesis using a plasmid that contains a region located upstream of the cmd gene in the genome of Neurospora crassa. This mutant is arrhythmic with regard to conidiation in continuous darkness but rhythmic under a light-dark cycle. After plasmid rescue from genomic DNA of the rhy-2 strain, the insertion was localized to the gene white collar-1 ( wc-1). Plasmid DNA was inserted 3' to the sequence encoding the polyglutamine region of the WC-1 gene product, and an mRNA encoding a truncated WC-1 protein must be synthesized under the control of the cmd promoter. The new wc-1 mutant, rhy-2, is still sensitive to light, although only weakly. Since the circadian rhythm of conidiation in continuous darkness is eliminated in the mutant, the polyglutamine region in WC-1 may be essential for both clock function and light-induced carotenogenesis in Neurospora.

MeSH terms

  • Alleles
  • Carotenoids / biosynthesis
  • Circadian Rhythm / radiation effects*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Darkness
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genes, Fungal / genetics
  • Light*
  • Mutation / genetics
  • Neurospora crassa / genetics*
  • Neurospora crassa / metabolism
  • Neurospora crassa / radiation effects*
  • Phenotype
  • Time Factors
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Transcription Factors
  • wc-1 protein, Neurospora crassa
  • Carotenoids