The HtrA1 serine protease is down-regulated during human melanoma progression and represses growth of metastatic melanoma cells

Oncogene. 2002 Sep 26;21(43):6684-8. doi: 10.1038/sj.onc.1205911.


Differential gene expression of cell lines derived from a malignant melanoma or its autologous lymph node metastasis using cDNA arrays indicated down-regulation of PRSS11, a gene encoding the serine protease HtrA1, a homolog of the Escherichia coli protease HtrA, in the metastatic line. Stable PRSS11 overexpression in the metastatic cell line strongly inhibited proliferation, chemoinvasion and Nm23-H1 protein expression in vitro, as well as cell growth in vivo in nu/nu mice. A polyclonal anti-HtrA1 serum demonstrated a significantly higher expression in primary melanomas when compared to unrelated metastatic lesions in a human melanoma tissue array, and down-modulation of HtrA1 expression in autologous lymph node melanoma metastases in seven out of 11 cases examined. These results suggest that down-regulation of PRSS11 and HtrA1 expression may represent an indicator of melanoma progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Mapping
  • Down-Regulation
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Immunohistochemistry
  • Melanoma / enzymology*
  • Melanoma / pathology
  • Melanoma / secondary
  • Rabbits
  • Serine Endopeptidases / analysis
  • Serine Endopeptidases / genetics*


  • Serine Endopeptidases