Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6

Yeast. 2002 Oct;19(14):1261-76. doi: 10.1002/yea.916.


In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1-3 and SNO1-3. In media lacking vitamin B(6), SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B(6) is essential for growth, copies 2 and 3 seem more related with B(1) biosynthesis during the exponential phase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Culture Media
  • Gene Expression Regulation, Fungal*
  • Multigene Family / physiology*
  • Pyridoxal
  • RNA, Fungal / analysis
  • RNA, Messenger / analysis
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Thiamine / biosynthesis
  • Thiamine / metabolism*
  • Two-Hybrid System Techniques
  • Vitamin B 6 / biosynthesis
  • Vitamin B 6 / metabolism*
  • Vitamin K 3


  • Culture Media
  • RNA, Fungal
  • RNA, Messenger
  • SNZ1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Pyridoxal
  • Vitamin K 3
  • Vitamin B 6
  • Thiamine