The hydration of proteoglycans of bovine cornea

Biochim Biophys Acta. 1975 Jan 13;381(1):203-14. doi: 10.1016/0304-4165(75)90202-0.


The water sorptive and retentive capacities of three corneal proteoglycans with different keratan sulfate/chondroitin-4-sulfate compositions were investigated. The calcium salt of a predominantly keratan sulfate containing proteoglycan had hydration properties similar to that of calcium keratan sulfate. The proteoglycan containing predominantly calcium chondroitin-4-sulfate side chains sorbed water to a greater extent than pure calcium chondroitin-4-sulfate but its retentive power was somewhat less. The proteoglycan containing about twice as much keratan sulfate as chondroitin-4-sulfate, on a dissaccharidic molar basis and had hydration properties which were closer to the behavior of chondroitin-4-sulfate than keratan sulfate. The results are discussed in terms of structure and polymer interaction in the proteoglycan matrices.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Calcium
  • Carbohydrates / analysis
  • Cattle
  • Chondroitin / analysis
  • Chromatography, Ion Exchange
  • Cornea / analysis*
  • Glycosaminoglycans*
  • Keratins / analysis
  • Protein Binding
  • Proteoglycans* / analysis
  • Water


  • Amino Acids
  • Carbohydrates
  • Glycosaminoglycans
  • Proteoglycans
  • Water
  • Keratins
  • Chondroitin
  • Calcium