Characterization of a general amino acid permease from Hebeloma cylindrosporum

FEBS Lett. 2002 Sep 25;528(1-3):119-24. doi: 10.1016/s0014-5793(02)03271-4.


Besides a role in phosphate supply, ectomycorrhizas play a crucial role in nitrogen nutrition of plants. The ectomycorrhizal association between Hebeloma cylindrosporum and Pinus pinaster serves as a model system accessible to molecular manipulation. Hebeloma mycelium is able to take up and use amino acids as the sole nitrogen source. Suppression cloning allowed identification of a Hebeloma transporter (HcGAP1) mediating histidine uptake. HcGAP1 mediates secondary active uptake of a wide spectrum of different amino acids. The secondary active transport mechanism together with the expression in hyphae, but not in mycorrhizas, indicate a role in uptake of organic nitrogen from the soil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / enzymology*
  • Agaricales / genetics
  • Agaricales / growth & development
  • Amino Acid Transport Systems / chemistry*
  • Amino Acid Transport Systems / genetics
  • Amino Acid Transport Systems / metabolism*
  • Amino Acids / metabolism
  • Aspartic Acid / metabolism
  • Biological Transport, Active
  • Cloning, Molecular
  • Genes, Fungal
  • Models, Molecular
  • Molecular Sequence Data
  • Nitrogen / metabolism
  • Phylogeny
  • Symbiosis
  • Trees / metabolism
  • Trees / microbiology


  • Amino Acid Transport Systems
  • Amino Acids
  • Aspartic Acid
  • Nitrogen

Associated data

  • GENBANK/AF521906