Enhancement of human papillomavirus (HPV) type 16 E6 and E7-specific T-cell immunity in healthy volunteers through vaccination with TA-CIN, an HPV16 L2E7E6 fusion protein vaccine

Vaccine. 2002 Oct 4;20(29-30):3456-64. doi: 10.1016/s0264-410x(02)00350-x.


TA-CIN is a vaccine that comprises the human papillomavirus (HPV) type 16 L2, E6 and E7 as a single fusion protein. In a mouse model, TA-CIN effectively prevented outgrowth of HPV16-positive tumour cells. To assess the safety and immunogenicity of TA-CIN, a dose escalating (26, 128, 533 micro g), double blind and placebo-controlled phase I study was conducted in 40 healthy volunteers. TA-CIN was administered without adjuvant by intramuscular injection on weeks 0, 4 and 8. No serious adverse events of the vaccination were reported during the study. Both IgG antibodies and proliferative responses against TA-CIN were elicited at all three doses. More importantly, T-cell immunity against the HPV16 E6 and E7 oncoproteins was detected by IFN gamma ELISPOT in 8/11 evaluable subjects vaccinated with the 533 micro g dose.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Viral / blood
  • Capsid / immunology*
  • Capsid Proteins*
  • Female
  • Humans
  • Immunization
  • Immunoglobulin G / blood
  • Male
  • Middle Aged
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / immunology*
  • Papillomavirus E7 Proteins
  • Papillomavirus Vaccines*
  • Repressor Proteins*
  • T-Lymphocytes / immunology*
  • Vaccination
  • Vaccines, Synthetic / immunology*
  • Viral Vaccines / adverse effects
  • Viral Vaccines / immunology*


  • Antibodies, Viral
  • Capsid Proteins
  • E6 protein, Human papillomavirus type 16
  • Immunoglobulin G
  • L2 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • Papillomavirus Vaccines
  • Repressor Proteins
  • Vaccines, Synthetic
  • Viral Vaccines
  • oncogene protein E7, Human papillomavirus type 16