An Escherichia coli-based oral vaccine against urinary tract infections potently activates human dendritic cells

Urology. 2002 Sep;60(3):521-6. doi: 10.1016/s0090-4295(02)01767-3.


Objectives: To investigate the effects of Uro-Vaxom, an oral vaccine against Escherichia coli urinary tract infections, on human monocyte-derived dendritic cells (moDCs). Dendritic cells (DCs) are important antigen-presenting cells of the immune system. DCs are considered promising cellular adjuvants for inducing immunity against cancer or infectious diseases.

Methods: moDCs were generated in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4. Flow cytometric phenotyping, as well as the ability to stimulate T cells in an allogeneic mixed leukocyte reaction, was used to assess the effects of Uro-Vaxom on human moDCs. In addition, interferon-gamma and interleukin-4 production by T cells stimulated with Uro-Vaxom-activated moDCs were measured by intracellular fluorescence-activated cell sorter-staining at the single-cell level.

Results: Uro-Vaxom induced the terminal maturation of CD83+ moDCs in a dose-dependent manner. Phenotypic analyses revealed that Uro-Vaxom-activated moDCs displayed a phenotype of mature DCs with high levels of MHC molecules and increased levels of co-stimulatory molecules (CD80, CD86). Consistent with these findings, Uro-Vaxom-activated moDCs potently stimulated T-cell proliferation and interferon-gamma production in the allogeneic mixed leukocyte reaction.

Conclusions: In DC-based immunotherapy, Uro-Vaxom could be used as a stimulant of DC maturation, which meets the standards of good manufacturing practice. In future preclinical studies, we will evaluate the effectiveness of a vaccination with Uro-Vaxom-activated moDCs. It could be an attractive treatment option in preventing recurrent E. coli urinary tract infections in predisposed individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Adjuvants, Immunologic / therapeutic use
  • Antigens, CD / immunology
  • Cells, Cultured
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Dose-Response Relationship, Immunologic
  • Escherichia coli / immunology
  • Escherichia coli Infections / immunology
  • Escherichia coli Infections / prevention & control
  • Escherichia coli Vaccines / pharmacology*
  • Escherichia coli Vaccines / therapeutic use
  • Flow Cytometry
  • Humans
  • Immunophenotyping
  • Interferon-gamma / immunology
  • Lymphocyte Culture Test, Mixed
  • Monocytes / immunology
  • Urinary Tract Infections / immunology
  • Urinary Tract Infections / prevention & control


  • Adjuvants, Immunologic
  • Antigens, CD
  • Escherichia coli Vaccines
  • Interferon-gamma