ASI1, a gene encoding a novel leucine zipper protein, is induced during development of the macronucleus in Tetrahymena

Mech Dev. 2002 Oct;118(1-2):215-8. doi: 10.1016/s0925-4773(02)00259-9.


Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb transcript that contains a single intron and is induced during macronuclear development. ASI1 is a single copy gene in both the micronucleus and the macronucleus. It encodes a 95 kDa conceptual protein with a leucine zipper near the amino terminus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chemotaxis
  • Gene Expression Regulation, Developmental*
  • Introns
  • Leucine Zippers*
  • Models, Genetic
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protozoan Proteins / biosynthesis*
  • Protozoan Proteins / genetics*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tetrahymena
  • Up-Regulation*


  • Protozoan Proteins
  • RNA, Messenger