The identification of a tetracycline resistance gene tet(M), on a Tn916-like transposon, in the Bacillus cereus group

FEMS Microbiol Lett. 2002 Sep 10;214(2):251-6. doi: 10.1111/j.1574-6968.2002.tb11355.x.


In order to investigate whether resistance genes present in bacteria in manure could transfer to indigenous soil bacteria, resistant isolates belonging to the Bacillus cereus group (Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis) were isolated from farm soil (72 isolates) and manure (12 isolates) samples. These isolates were screened for tetracycline resistance genes (tet(K), tet(L), tet(M), tet(O), tet(S) and tet(T)). Of 88 isolates examined, three (3.4%) isolates carried both tet(M) and tet(L) genes, while four (4.5%) isolates carried the tet(L) gene. Eighty-one (92.1%) isolates did not contain any of the tested genes. All tet(M) positive isolates carried transposon Tn916 and could transfer this mobile DNA element to other Gram-positive bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus cereus / drug effects
  • Bacillus cereus / genetics*
  • DNA Transposable Elements*
  • Gene Transfer, Horizontal
  • Microbial Sensitivity Tests
  • Soil Microbiology
  • Tetracycline Resistance / genetics*


  • DNA Transposable Elements