RGM is a repulsive guidance molecule for retinal axons

Nature. 2002 Sep 26;419(6905):392-5. doi: 10.1038/nature01041.


Axons rely on guidance cues to reach remote targets during nervous system development. A well-studied model system for axon guidance is the retinotectal projection. The retina can be divided into halves; the nasal half, next to the nose, and the temporal half. A subset of retinal axons, those from the temporal half, is guided by repulsive cues expressed in a graded fashion in the optic tectum, part of the midbrain. Here we report the cloning and functional characterization of a membrane-associated glycoprotein, which we call RGM (repulsive guidance molecule). This molecule shares no sequence homology with known guidance cues, and its messenger RNA is distributed in a gradient with increasing concentration from the anterior to posterior pole of the embryonic tectum. Recombinant RGM at low nanomolar concentration induces collapse of temporal but not of nasal growth cones and guides temporal retinal axons in vitro, demonstrating its repulsive and axon-specific guiding activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Avian Proteins / analysis
  • Avian Proteins / chemistry
  • Avian Proteins / genetics*
  • Avian Proteins / metabolism*
  • Axons / metabolism*
  • Base Sequence
  • Chick Embryo
  • Cloning, Molecular
  • Growth Cones / metabolism
  • Hydrophobic and Hydrophilic Interactions
  • In Situ Hybridization
  • Mass Spectrometry
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Retina / cytology*
  • Superior Colliculi / cytology
  • Superior Colliculi / metabolism


  • Avian Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AY128507