The spectrin-ankyrin skeleton controls CD45 surface display and interleukin-2 production

Immunity. 2002 Sep;17(3):303-15. doi: 10.1016/s1074-7613(02)00396-5.

Abstract

With T cell receptor stimulation, intracellular pools of CD45 and spectrin move to the surface. These processes are coupled. In both peripheral lymphocytes and Jurkat T cells, betaI spectrin and ankyrin associate with CD45. In Jurkat T cells, betaI spectrin peptides suppress surface recruitment of CD45 and CD3 and abrogate T cell activation. Other glycoproteins such as CD43 are not altered by the spectrin peptides. Spectrin's effects are mediated by ankyrin, which binds directly to the cytoplasmic domain of CD45 (K(d) = 4.3 +/- 3.0 nM). These data reveal a novel and unexpected contribution of the spectrin-ankyrin skeleton to the control of T lymphocyte function.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ankyrins / physiology*
  • Ankyrins / ultrastructure
  • CD3 Complex / metabolism
  • Cell Membrane / metabolism*
  • Humans
  • Interleukin-2 / biosynthesis*
  • Interleukin-2 / genetics
  • Interleukin-2 / metabolism
  • Jurkat Cells / immunology
  • Jurkat Cells / metabolism
  • Leukocyte Common Antigens / metabolism*
  • Lymphocyte Activation / physiology*
  • Macromolecular Substances
  • Membrane Glycoproteins / metabolism*
  • Neoplasm Proteins / physiology
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Isoforms / physiology
  • Protein Isoforms / ultrastructure
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / physiology
  • Spectrin / chemistry
  • Spectrin / physiology*
  • Spectrin / ultrastructure
  • Structure-Activity Relationship
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Transfection

Substances

  • Ankyrins
  • CD3 Complex
  • Interleukin-2
  • Macromolecular Substances
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Peptide Fragments
  • Protein Isoforms
  • Recombinant Fusion Proteins
  • SPTBN1 protein, human
  • Spectrin
  • Leukocyte Common Antigens