Altered presynaptic vesicle release and cycling during mGluR-dependent LTD

Neuron. 2002 Sep 12;35(6):1099-110. doi: 10.1016/s0896-6273(02)00898-x.


The site of modification of synaptic transmission during long-term plasticity in the mammalian hippocampus remains controversial. Here we used a fluorescent marker of presynaptic activity, FM 1-43, to directly image presynaptic function during metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) at CA3-CA1 excitatory synapses in acute hippocampal slices. We found a significant decrease in the rate of FM 1-43 release in response to synaptic stimulation following induction of mGluR-LTD, providing direct evidence for altered presynaptic function. Moreover, we found that mGluR-LTD causes several changes in FM dye release properties that are consistent with a change in the mode of vesicle cycling, possibly involving a switch from a full fusion mode of release to a "kiss-and-run" mode of release through the transient opening of a fusion pore.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Electric Stimulation
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • Exocytosis / physiology
  • Hippocampus / metabolism*
  • Hippocampus / ultrastructure
  • Ion Channels / metabolism
  • Mice
  • Neural Inhibition / physiology*
  • Neuronal Plasticity / physiology*
  • Presynaptic Terminals / metabolism*
  • Presynaptic Terminals / ultrastructure
  • Protein Transport / physiology
  • Receptors, Metabotropic Glutamate / metabolism*
  • Synaptic Membranes / metabolism*
  • Synaptic Membranes / ultrastructure
  • Synaptic Transmission / physiology
  • Synaptic Vesicles / metabolism*
  • Synaptic Vesicles / ultrastructure


  • Ion Channels
  • Receptors, Metabotropic Glutamate