In-situ chemical analyses of trans-polyisoprene by histochemical staining and Fourier transform infrared microspectroscopy in a rubber-producing plant, Eucommia ulmoides Oliver

Planta. 2002 Oct;215(6):934-9. doi: 10.1007/s00425-002-0832-3. Epub 2002 Jul 25.

Abstract

The localization of polyisoprene in young stem tissues of Eucommia ulmoides Oliver was investigated by histochemical staining and Fourier transform infrared (FT-IR) microspectroscopy. The fibrous structures were stained with Oil Red O. FT-IR microspectroscopic analysis proved that the fibrous structures were trans-polyisoprene. Granular structures stained with the dye, and characteristic absorptions at 2,960 cm(-1) and 1,430 cm(-1) in FT-IR suggested that trans-polyisoprene accumulated in the vicinity of the cambium layer. We have thus successfully shown for the first time the localization of trans-polyisoprene in plant tissues, and our histological investigation allowed us to presume the main sites of biosynthesis and accumulation of trans-rubber. Furthermore, a new technical approach, the preparation of sections using an electronic freezing unit and the in situ analysis of polyisoprene using FT-IR microspectroscopy, is demonstrated to be a promising method for determining the accumulation of polyisoprene as well as other metabolites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Butadienes / metabolism*
  • Eucommiaceae / chemistry*
  • Eucommiaceae / metabolism
  • Hemiterpenes*
  • Histocytochemistry / methods*
  • Pentanes*
  • Plant Bark / chemistry
  • Plant Bark / metabolism
  • Plant Leaves / chemistry
  • Plant Leaves / metabolism
  • Plant Stems / chemistry
  • Plant Stems / metabolism
  • Rubber / metabolism*
  • Spectroscopy, Fourier Transform Infrared / methods*

Substances

  • Azo Compounds
  • Butadienes
  • Hemiterpenes
  • Pentanes
  • isoprene
  • Rubber
  • oil red O