Method for determining nitrogenous heterocycle compounds in wine

J Agric Food Chem. 2002 Oct 9;50(21):5803-7. doi: 10.1021/jf025591m.


Qualitative and quantitative analyses of N-heterocycle compounds were conducted by using a liquid-liquid extraction followed by additional chemical silica gel purification and injection into a gas chromatographic (GC) column coupled to a nitrogen-phosphorus detector (NPD). The purified extract fraction was investigated by GC-NPD and by GC-mass spectrometry. The compounds identified and quantified in wines were 2,4,5-trimethyloxazole, 2,4-dimethylthiazole, and 4-methylthiazole by GC-NPD and both 2-acetylthiazole and 2-acetyl-2-thiazoline by GC-MS. The procedure was used to analyze different wines and gave reliable and reproducible results.

MeSH terms

  • Chromatography, Gas
  • Gas Chromatography-Mass Spectrometry
  • Heterocyclic Compounds / analysis*
  • Hydrogen-Ion Concentration
  • Quality Control
  • Reproducibility of Results
  • Thiazoles / analysis*
  • Wine / analysis*


  • Heterocyclic Compounds
  • Thiazoles