Preparation of microparticulate beta-glucan from Saccharomyces cerevisiae for use in immune potentiation

Lett Appl Microbiol. 2002;35(4):267-71. doi: 10.1046/j.1472-765x.2002.01201.x.


Aims: To develop a method for the preparation of an immunologically active, homogeneous, nonaggregated, microparticulate beta-glucan-containing material from the budding yeast Saccharomyces cerevisiae.

Methods and results: Using a combination of sonication and spray-drying, a homogeneous preparation of 1-2-mu diameter beta-glucan-containing particles was made from alkali- and acid-insoluble yeast cell wall material. This microparticulate beta-glucan remained in suspension longer and, following oral administration at 0.1 mg kg(-1) for 14 d, enhanced phagocytosis of mouse peritoneal macrophages significantly better than did aggregated beta-glucan particles.

Conclusions: A new sonication and spray-drying method can be employed to overcome the problem of aggregation of beta-glucan microparticles in aqueous media.

Significance and impact of the study: A microparticulate form of beta-glucan that remains in suspension longer for pharmaceutical applications and has superior immune potentiation characteristics has been developed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / isolation & purification*
  • Adjuvants, Immunologic / pharmacology
  • Animals
  • Glucans / immunology
  • Glucans / isolation & purification*
  • Glucans / pharmacology
  • In Vitro Techniques
  • Macrophages / drug effects
  • Macrophages / immunology
  • Mice
  • Mice, Inbred BALB C
  • Phagocytosis / drug effects
  • Reagent Kits, Diagnostic
  • Saccharomyces cerevisiae / chemistry*
  • Sonication


  • Adjuvants, Immunologic
  • Glucans
  • Reagent Kits, Diagnostic