A role for survivin in radioresistance of pancreatic cancer cells

Jpn J Cancer Res. 2002 Sep;93(9):1057-62. doi: 10.1111/j.1349-7006.2002.tb02483.x.


Using gene-transduced pancreatic cancer cells, we examined whether survivin expression is directly involved in regulation of radiosensitivity. Ordinarily radiosensitive MIAPaCa-2 cells transduced with wild-type survivin gene (MS cells) proliferated more rapidly than cells transduced with control vector. MS cells were significantly less radiosensitive than control vector-transduced cells. Radiation-induced activity of caspase-3, but not caspase-7, was significantly inhibited in MS cells. On the other hand, transduction of a dominant-negative mutant survivin gene into radioresistant PANC-1 cells augmented radiosensitivity. Further, the radiation-induced increase in caspase-3 activity was enhanced, indicating that survivin function was truly inhibited. These results indicate that survivin expression directly down-regulates radiosensitivity.

MeSH terms

  • Caspase 3
  • Caspases / metabolism
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / physiology*
  • Neoplasm Proteins
  • Pancreatic Neoplasms / enzymology
  • Pancreatic Neoplasms / radiotherapy*
  • Radiation Tolerance*
  • Survivin
  • Transduction, Genetic
  • Tumor Cells, Cultured
  • X-Rays


  • BIRC5 protein, human
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Survivin
  • CASP3 protein, human
  • Caspase 3
  • Caspases