Association Between the Cell Cycle and Neural Crest Delamination Through Specific Regulation of G1/S Transition

Dev Cell. 2002 Sep;3(3):383-95. doi: 10.1016/s1534-5807(02)00221-6.

Abstract

Delamination of premigratory neural crest cells from the dorsal neural tube depends both upon environmental signals and cell-intrinsic mechanisms and is a prerequisite for cells to engage in migration. Here we show that avian neural crest cells synchronously emigrate from the neural tube in the S phase of the cell cycle. Furthermore, specific inhibition of the transition from G1 to S both in ovo and in explants blocks delamination, whereas arrest at the S or G2 phases has no immediate effect. Thus, the events taking place during G1 that control the transition from G1 to S are necessary for the epithelial to mesenchymal conversion of crest precursors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimetabolites / pharmacology
  • Bromodeoxyuridine / pharmacology
  • Cell Cycle / physiology
  • Cell Differentiation
  • Cell Movement
  • Cell Nucleus / physiology
  • Chick Embryo
  • Culture Techniques
  • DNA / biosynthesis
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • G1 Phase / physiology
  • Kinetin
  • Mimosine / pharmacology
  • Models, Biological
  • Morphogenesis
  • MyoD Protein / chemistry
  • MyoD Protein / metabolism
  • Neural Crest / cytology
  • Neural Crest / embryology*
  • Neural Crest / physiology
  • Purines / pharmacology
  • Quail / embryology
  • S Phase / physiology
  • Transcription Factors / metabolism
  • Tyrphostins / pharmacology

Substances

  • Antimetabolites
  • Enzyme Inhibitors
  • MyoD Protein
  • Purines
  • Transcription Factors
  • Tyrphostins
  • alpha-cyano-(3,4-dihydroxy)-N-(3-phenylpropyl)cinnamide
  • Mimosine
  • olomoucine
  • DNA
  • Bromodeoxyuridine
  • Kinetin