Lumenal Transmission of Decapentaplegic in Drosophila Imaginal Discs

Dev Cell. 2002 Sep;3(3):451-60. doi: 10.1016/s1534-5807(02)00264-2.

Abstract

Drosophila imaginal discs are sac-like appendage primordia comprising apposed peripodial and columnar cell layers. Cell survival in disc columnar epithelia requires the secreted signal Decapentaplegic (DPP), which also acts as a gradient morphogen during pattern formation. The distribution mechanism by which secreted DPP mediates global cell survival and graded patterning is poorly understood. Here we report detection of DPP in the lumenal cavity between apposed peripodial and columnar cell layers of both wing and eye discs. We show that peripodial cell survival hinges upon DPP signal reception and implicate DPP-dependent viability of the peripodial epithelium in growth of the entire disc. These results are consistent with lumenal transmission of the DPP survival signal during imaginal disc development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning
  • Cell Size
  • Cell Survival
  • Drosophila / genetics
  • Drosophila / growth & development*
  • Drosophila Proteins / physiology*
  • Embryonic Development
  • Epithelial Cells / cytology
  • Eye / cytology
  • Eye / growth & development
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins
  • Larva / growth & development
  • Luminescent Proteins / metabolism
  • Metamorphosis, Biological
  • Models, Biological
  • Morphogenesis
  • Mutation
  • Receptors, Cell Surface / physiology
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction*
  • Transforming Growth Factor beta / physiology
  • Wings, Animal / cytology
  • Wings, Animal / growth & development

Substances

  • Drosophila Proteins
  • Luminescent Proteins
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Transforming Growth Factor beta
  • dpp protein, Drosophila
  • Green Fluorescent Proteins