Proteomic analysis of rice leaves during drought stress and recovery

Proteomics. 2002 Sep;2(9):1131-45. doi: 10.1002/1615-9861(200209)2:9<1131::AID-PROT1131>3.0.CO;2-1.


Three-week old plants of rice (Oryza sativa L. cv CT9993 and cv IR62266) developed gradual water stress over 23 days of transpiration without watering, during which period the mid-day leaf water potential declined to approximately -2.4 MPa, compared with approximately -1.0 MPa in well-watered controls. More than 1000 protein spots that were detected in leaf extracts by proteomic analysis showed reproducible abundance within replications. Of these proteins, 42 spots showed a significant change in abundance under stress, with 27 of them exhibiting a different response pattern in the two cultivars. However, only one protein (chloroplast Cu-Zn superoxide dismutase) changed significantly in opposite directions in the two cultivars in response to drought. The most common difference was for proteins to be up-regulated by drought in CT9993 and unaffected in IR62266; or down-regulated by drought in IR62266 and unaffected in CT9993. By 10 days after rewatering, all proteins had returned completely or largely to the abundance of the well-watered control. Mass spectrometry helped to identify 16 of the drought-responsive proteins, including an actin depolymerizing factor, which was one of three proteins detectable under stress in both cultivars but undetectable in well-watered plants or in plants 10 days after rewatering. The most abundant protein up-regulated by drought in CT9993 and IR62266 was identified only after cloning of the corresponding cDNA. It was found to be an S-like RNase homologue but it lacked the two active site histidines required for RNase activity. Four novel drought-responsive mechanisms were revealed by this work: up-regulation of S-like RNase homologue, actin depolymerizing factor and rubisco activase, and down-regulation of isoflavone reductase-like protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Depolymerizing Factors
  • Amino Acid Sequence
  • Base Sequence
  • Citrullus / chemistry
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Databases as Topic
  • Destrin
  • Disasters
  • Electrophoresis, Gel, Two-Dimensional
  • Genotype
  • Image Processing, Computer-Assisted
  • Mass Spectrometry
  • Microfilament Proteins / metabolism
  • Molecular Sequence Data
  • Oryza / chemistry*
  • Peptides / chemistry
  • Phylogeny
  • Plant Leaves / chemistry*
  • Plant Proteins
  • Proteome
  • Ribonucleases / metabolism
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Time Factors
  • Water / metabolism
  • Zea mays / chemistry


  • Actin Depolymerizing Factors
  • DNA, Complementary
  • Destrin
  • Microfilament Proteins
  • Peptides
  • Plant Proteins
  • Proteome
  • rca protein, plant
  • Water
  • Ribonucleases
  • S-like RNase protein, plant