Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12

Mol Microbiol. 2002 Oct;46(1):281-91. doi: 10.1046/j.1365-2958.2002.03170.x.


We have systematically examined the mRNA profiles of 36 two-component deletion mutants, which include all two-component regulatory systems of Escherichia coli, under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria-Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two-component systems. A high correlation coefficient of expression profile was found between several two-component mutants. Together, these results support the view that a network of functional interactions, such as cross-regulation, exists between different two-component systems. The compiled data are avail-able at our website ( 2_components).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Escherichia coli / physiology
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial*
  • Mutation*
  • Oligonucleotide Array Sequence Analysis
  • Proteome
  • Signal Transduction*
  • Transcription, Genetic


  • Bacterial Proteins
  • Proteome