The determination of levofloxacin by flow injection analysis using UV detection, potentiometry, and conductometry in pharmaceutical preparations

J Pharm Biomed Anal. 2002 Oct 15;30(3):881-5. doi: 10.1016/s0731-7085(02)00354-0.

Abstract

A flow injection analysis (FIA) using UV detection, potentiometry and conductometry for levofloxacin (LVF) are described in this study. The best solvent system was found to consist of 0.2 M acetate buffer at pH 3 having 10% MeOH. A flow rate of 1 ml min(-1) was pumped and active material was detected at 288 nm. The detection limit (LOD) and limit of quantification (LOQ) for FIA were calculated to be 3 x 10(-7) M (S/N = 3) and 1 x 10(-7) M (S/N = 10), respectively. In the analysis of tablets, the RSD values were found to be 0.83, 0.98 and 0.99 for FIA, potentiometric and conductometric methods, respectively.

MeSH terms

  • Conductometry / methods
  • Flow Injection Analysis / methods
  • Levofloxacin*
  • Ofloxacin / analysis*
  • Ofloxacin / chemistry
  • Pharmaceutical Preparations / analysis
  • Pharmaceutical Preparations / chemistry
  • Potentiometry / methods
  • Spectrophotometry, Ultraviolet / methods

Substances

  • Pharmaceutical Preparations
  • Levofloxacin
  • Ofloxacin