Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis

Nat Biotechnol. 2002 Nov;20(11):1118-23. doi: 10.1038/nbt749. Epub 2002 Oct 7.


Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803-base pair circular chromosome with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613-base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron transport system. This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion-reducing capabilities.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Biodegradation, Environmental
  • Cell Respiration
  • Electron Transport
  • Gene Expression
  • Gene Expression Regulation, Bacterial*
  • Genome, Bacterial*
  • Metals / metabolism
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Organic Chemicals / metabolism
  • Oxidation-Reduction
  • Plasmids
  • Proteomics / methods
  • Sequence Alignment / methods
  • Sequence Analysis, DNA*
  • Sequence Analysis, Protein*
  • Shewanella / classification
  • Shewanella / genetics*
  • Shewanella / metabolism*
  • Shewanella / pathogenicity
  • Species Specificity
  • Water Pollutants, Chemical / metabolism
  • Water Purification / methods


  • Metals
  • Organic Chemicals
  • Water Pollutants, Chemical

Associated data

  • GENBANK/AE014299
  • GENBANK/AE014300