Effects of leptin, troglitazone, and dietary fat on stearoyl CoA desaturase

Biochem Biophys Res Commun. 2002 Oct 11;297(5):1259-63. doi: 10.1016/s0006-291x(02)02375-6.

Abstract

Leptin, troglitazone, and high fat feeding profoundly influence the lipid content of various tissues. To determine if they affect the expression of stearoyl CoA desaturase (SCD)-1 and -2, their mRNA was measured in livers of normal, hyperleptinemic, troglitazone-treated, and fat-fed rats. Hyperleptinemia, which reduces tissue TG by downregulating lipogenic enzymes and upregulating fatty acid oxidation, lowered SCD-1 96% below controls and reduced SCD-2 slightly. By contrast, hepatic SCD-1 mRNA of leptin-resistant fa/fa rats was five times wild-type controls, but SCD-2 mRNA was 66% lower. High fat feeding lowered SCD-1 by 80%, possibly by inducing hyperleptinemia. Troglitazone treatment, which reduces nonadipose tissue TG of fa/fa rats without downregulating lipogenic enzymes, raised SCD-2 13-fold but lowered SCD-1 by 25%. The findings suggest that leptin controls SCD-1 expression and that troglitazone's antilipotoxic action may involve SCD-2 upregulation.

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Antioxidants / pharmacology
  • Chromans / pharmacology*
  • DNA, Complementary / metabolism
  • Dietary Fats / pharmacology*
  • Down-Regulation
  • Fats / metabolism
  • Leptin / pharmacology*
  • Linear Models
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Polymerase Chain Reaction
  • RNA / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Zucker
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stearoyl-CoA Desaturase / chemistry
  • Stearoyl-CoA Desaturase / metabolism*
  • Thiazoles / pharmacology*
  • Thiazolidinediones*
  • Troglitazone
  • Up-Regulation

Substances

  • Antioxidants
  • Chromans
  • DNA, Complementary
  • Dietary Fats
  • Fats
  • Leptin
  • RNA, Messenger
  • Thiazoles
  • Thiazolidinediones
  • RNA
  • Stearoyl-CoA Desaturase
  • Troglitazone