WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

FEBS Lett. 2002 Oct 9;529(2-3):319-24. doi: 10.1016/s0014-5793(02)03360-4.

Abstract

Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • CDC2 Protein Kinase / metabolism*
  • Chromatography, Gel
  • Cyclin B / metabolism*
  • Drosophila Proteins*
  • Genes, Tumor Suppressor*
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence
  • Mitosis*
  • Phosphorylation
  • Precipitin Tests
  • Protein Kinases*
  • Protein-Serine-Threonine Kinases / immunology
  • Protein-Serine-Threonine Kinases / metabolism*
  • Spindle Apparatus*
  • Subcellular Fractions / metabolism
  • Substrate Specificity

Substances

  • CycB protein, Drosophila
  • Cyclin B
  • Drosophila Proteins
  • Protein Kinases
  • LATS1 protein, human
  • wts protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • CDC2 Protein Kinase