Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritis

Electrophoresis. 2002 Sep;23(19):3445-56. doi: 10.1002/1522-2683(200210)23:19<3445::AID-ELPS3445>3.0.CO;2-J.


Differential proteome analysis is used to study body fluids from patients suffering from rheumatoid arthritis (RA), reactive arthritis (reaA) or osteoarthritis (OA). Mass spectrometric structure characterization of gel-separated proteins provided a detailed view of the protein-processing events that lead to distinct protein species present in the respective body fluids. (i) Fibrin(ogen) beta-chain degradation products, presumably plasmin-derived, appeared solely in synovial fluids (SF) from both patient collectives, (ii) calgranulin B (MRP14) was exclusively identified in SF samples derived from 5 out of 6 patients suffering from RA. Calgranulin B was not observed in synovial fluids from OA patients, nor in plasmas from either patient group. In all cases where calgranulin B was detected, calgranulin C was identified as well. (iii) Serum amyloid A protein spots were determined in plasmas and synovial fluids from patients with RA, but not in patients with OA. In addition to disease-relevant differences, interindividual differences in haptoglobin patterns of the patients under investigation were observed. Hence, in-depth proteome analysis of body fluids has proven effective for identification of multiple molecular markers and determination of associated protein structure modifications, that are thought to play a role for specifically determining a defined pathological state of diseased joints.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Amino Acid Sequence
  • Arthritis, Reactive / blood
  • Arthritis, Reactive / metabolism*
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / metabolism*
  • Electrophoresis, Gel, Two-Dimensional
  • Fibrinogen / chemistry
  • Fibrinogen / metabolism
  • Humans
  • Middle Aged
  • Molecular Weight
  • Osteoarthritis / blood
  • Osteoarthritis / metabolism*
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism
  • Plasma / chemistry*
  • Proteome / analysis*
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Synovial Fluid / chemistry*


  • Peptide Fragments
  • Proteome
  • Fibrinogen