Nogo-receptor gene activity: cellular localization and developmental regulation of mRNA in mice and humans

J Comp Neurol. 2002 Nov 18;453(3):292-304. doi: 10.1002/cne.10408.


Nogo (reticulon-4) is a myelin-associated protein that is expressed in three different splice variants, Nogo-A, Nogo-B, and Nogo-C. Nogo-A inhibits neurite regeneration in the central nervous system. Messenger RNA encoding Nogo is expressed in oligodendrocytes and central and peripheral neurons, but not in astrocytes or Schwann cells. Nogo is a transmembraneous protein; the extracellular domain is termed Nogo-66, and a Nogo-66-receptor (Nogo-R) has been identified. We performed in situ hybridization in human and mouse nervous tissues to map the cellular distribution of Nogo-R gene activity patterns in fetal and adult human spinal cord and sensory ganglia, adult human brain, and the nervous systems of developing and adult mice. In the human fetus Nogo-R was transcribed in the ventral horn of the spinal cord and in dorsal root ganglia. In adult human tissues Nogo-R gene activity was found in neocortex, hippocampus, amygdala, and a subset of large and medium-sized neurons of the dorsal root ganglia. Nogo-R mRNA was not expressed in the adult human spinal cord at detectable levels. In the fetal mouse, Nogo-R was diffusely expressed in brain, brainstem, trigeminal ganglion, spinal cord, and dorsal root ganglia at all stages. In the adult mouse strong Nogo-R mRNA expression was found in neurons in neocortex, hippocampus, amygdala, habenula, thalamic nuclei, brainstem, the granular cell layer of cerebellum, and the mitral cell layer of the olfactory bulb. Neurons in the adult mouse striatum, the medial septal nucleus, and spinal cord did not express Nogo-R mRNA at detectable levels. In summary, Nogo-66-R mRNA expression in humans and mice was observed in neurons of the developing nervous system Expression was downregulated in the adult spinal cord of both species, and specific expression patterns were seen in the adult brain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Animals
  • Fetus
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / embryology
  • Gene Expression Regulation, Developmental*
  • Hippocampus / cytology
  • Hippocampus / embryology
  • Humans
  • In Situ Hybridization
  • Intracellular Signaling Peptides and Proteins*
  • Membrane Proteins*
  • Mice
  • Mice, Inbred C57BL / physiology*
  • Myelin Proteins / genetics*
  • Nerve Regeneration / physiology
  • Nerve Tissue Proteins / genetics
  • Neurons / physiology
  • Nogo Proteins
  • RNA, Messenger / analysis
  • Spinal Cord / cytology
  • Spinal Cord / embryology


  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Myelin Proteins
  • Nerve Tissue Proteins
  • Nogo Proteins
  • RNA, Messenger
  • RTN4 protein, human
  • Rtn4 protein, mouse