Characterization of nucleic acids by nanopore analysis

Acc Chem Res. 2002 Oct;35(10):817-25. doi: 10.1021/ar000138m.


Single-stranded DNA and RNA molecules in solution can be driven through a nanoscopic pore by an applied electric field. As each molecule occupies the pore, a characteristic blockade of ionic current is produced. Information about length, composition, structure, and dynamic motion of the molecule can be deduced from modulations of the current blockade.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Biopolymers / chemistry
  • DNA, Single-Stranded / chemistry
  • Electric Conductivity
  • Electrochemistry / methods*
  • Hemolysin Proteins / chemistry
  • Nanotechnology / methods*
  • Nucleic Acid Conformation*
  • Nucleic Acids / chemistry*
  • Temperature


  • Biopolymers
  • DNA, Single-Stranded
  • Hemolysin Proteins
  • Nucleic Acids