Modulation of a heterologous immune response by the products of Ascaris suum

Infect Immun. 2002 Nov;70(11):6058-67. doi: 10.1128/IAI.70.11.6058-6067.2002.

Abstract

Helminth infections are among the most potent stimulators of Th2-type immune responses and have been widely demonstrated to modify responsiveness to both nonparasite antigens and other infectious agents in a nonspecific manner in infected animals. We investigated the immunomodulatory properties of pseudocoelomic body fluid from adult Ascaris suum gastrointestinal helminths (ABF) and its defined allergen (ABA-1) by examining their effects on the immune response to a heterologous antigen, ovalbumin. Our results indicate that ABF has potent immunomodulatory activity and that the effects observed are consistent with skewing towards a Th2-type response rather than induction of anergy. Our findings show that the immunomodulatory activities of ABF are associated with components other than the major constituent and putative allergen, ABA-1. Furthermore, the allergic responses to ABA-1 are not a result of an intrinsic allergenicity of the protein but are more a reflection of the wider induction of a Th2 response by the infection. Importantly, the induction of interleukin-10 by ABF also suggests that T regulatory cells may play a role in immunomodulation of immune responses by parasitic helminths.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens*
  • Animals
  • Antigens, Helminth / immunology*
  • Antigens, Plant
  • Ascaris suum / immunology*
  • Dose-Response Relationship, Immunologic
  • Female
  • Helminth Proteins / immunology*
  • Hypersensitivity, Delayed / etiology
  • Immunoglobulin E / blood
  • Immunoglobulin G / blood
  • Interferon-gamma / biosynthesis
  • Interleukin-10
  • Interleukin-4 / physiology
  • Major Histocompatibility Complex
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / immunology
  • T-Lymphocytes / immunology

Substances

  • ABA-1 protein, Ascaris suum
  • Allergens
  • Antigens, Helminth
  • Antigens, Plant
  • Helminth Proteins
  • Immunoglobulin G
  • Interleukin-10
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma
  • Ovalbumin