Frequency of Escherichia coli O157:H7 in Turkish cattle

J Food Prot. 2002 Oct;65(10):1637-40. doi: 10.4315/0362-028x-65.10.1637.


In this study, five abattoirs in Istanbul were visited between January 2000 and April 2001. During these visits, 330 cattle were selected by a systematic sampling method. Cattle were examined clinically and breed, age, and sex were recorded. Rectal swabs were taken immediately after slaughter. Immunomagnetic separation was performed, and sorbitol-negative colonies were selected on sorbitol MacConkey agar with cefixime and tellurite (CT-SMAC agar). These colonies were checked for 4-methylenebelliferyl-beta-D-glucuronide, indol, rhamnose, and urease activity and motility. Serotypes of bacteria were determined by using antisera specific for Escherichia coli O157 and H7. All cattle selected were clinically healthy. Of 88 sorbitol-negative colonies selected on CT-SMAC agar, isolates from only 14 (4.2%) cattle reacted with anti-O157, and 13 of these isolates also reacted with anti-H7. E. coli O157:H7 was isolated from all breeds, but the numbers of isolates were largest for Holstein and Swiss Brown cows. E. coli O157:H7 was most frequently isolated from 2-year-old cattle. Similarly, it was most frequently isolated from male cattle. E. coli O157:H7 was isolated from cattle slaughtered in four of the five abattoirs studied.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abattoirs*
  • Age Factors
  • Animals
  • Cattle
  • Cattle Diseases / epidemiology*
  • Cattle Diseases / microbiology
  • Cefixime
  • Culture Media
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Escherichia coli O157 / classification
  • Escherichia coli O157 / isolation & purification*
  • Female
  • Glucuronidase / metabolism
  • Immunomagnetic Separation / veterinary
  • Male
  • Prevalence
  • Sex Factors
  • Sorbitol / metabolism
  • Tellurium
  • Turkey / epidemiology


  • Culture Media
  • Sorbitol
  • Cefixime
  • Glucuronidase
  • Tellurium