High-throughput global peptide proteomic analysis by combining stable isotope amino acid labeling and data-dependent multiplexed-MS/MS

Anal Chem. 2002 Oct 1;74(19):4994-5000. doi: 10.1021/ac020105f.

Abstract

In this work, we describe the application of a stable isotope amino acid (lysine) labeling in conjunction with data-dependent multiplexed tandem mass spectrometry (MS/MS) to facilitate the characterization and identification of peptides from proteomic (global protein) digests. Lysine auxotrophic yeast was grown in the presence of 13C-labeled or unlabeled lysine and combined after harvesting in equal proportions. Endoproteinase LysC digestion of the cytosolic fraction produced a global proteomic sample, consisting of heavy/light labeled peptide pairs. Then data-dependent multiplexed-MS/MS was applied to simultaneously select and dissociate only labeled peptide ion pairs. The approach allows differentiation between N-terminal (e.g., b-type ions) and C-terminal fragment ions (e.g., y-type ions) in resulting tandem mass spectra, as well as the capability of differentiation between near-isobaric glutamine and lysine residues. We also describe the utility of peptide composition and fragment information to support peptide identifications and examine the potential application of lysine labeling for differential quantitative protein analysis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis*
  • Chromatography, Micellar Electrokinetic Capillary
  • Cytosol / chemistry
  • Cytosol / metabolism
  • Databases, Factual
  • Endopeptidases / chemistry
  • Mass Spectrometry
  • Peptides / analysis*
  • Protein Hydrolysates / chemistry
  • Saccharomyces cerevisiae / chemistry

Substances

  • Amino Acids
  • Peptides
  • Protein Hydrolysates
  • Endopeptidases