Abstract
Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.
MeSH terms
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Ammonium Sulfate
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Animals
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Avidin / pharmacology
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Brain / enzymology*
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Chemical Precipitation
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Enzyme Activation
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Kinetics
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Liver / enzymology
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Male
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Mitochondria / enzymology
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Molecular Weight
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Polyethylene Glycols
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Pyruvate Carboxylase / antagonists & inhibitors
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Pyruvate Carboxylase / isolation & purification*
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Rats
Substances
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Avidin
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Polyethylene Glycols
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Pyruvate Carboxylase
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Ammonium Sulfate