TPA stimulation culture for improved detection of t(11;14)(q13;q32) in mantle cell lymphoma

Ann Genet. 2002 Jul-Sep;45(3):165-8. doi: 10.1016/s0003-3995(02)01122-x.

Abstract

Cytogenetic analysis of mantle cell lymphoma (MCL), characterized by the presence of t(11;14)(q13;q32) translocation, is often difficult because of the low proliferating rate of MCL cells and the presence of normal cells in bone marrow which may interfere with growth of MCL cells. We describe herein a TPA (12-O-tetradecanoylphorbol 13-acetate) stimulated culture to improve detection of t(11;14)(q13;q32) in 20 MCL patients regardless of the samples used.

MeSH terms

  • Adult
  • Aged
  • Cell Culture Techniques / methods
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 14*
  • Female
  • Humans
  • Lymphoma, Mantle-Cell / diagnosis
  • Lymphoma, Mantle-Cell / genetics*
  • Male
  • Middle Aged
  • Tetradecanoylphorbol Acetate / pharmacology
  • Translocation, Genetic*
  • Tumor Cells, Cultured

Substances

  • Tetradecanoylphorbol Acetate