Abstract
We report the first description of the microbial community in a stream of acidic hydrothermal waters on volcanically active White Island, New Zealand, using both molecular and microbiological methods. alpha- and beta-Proteobacteria, green-sulfur bacteria, and uncultured Firmicutes were identified from the community DNA-based 16s rRNA gene library. The same bacterial groups and the Rhodophyte Cyanidium caldarium were represented in enrichment cultures. C. caldarium, two Firmicutes and an acidophilic alpha-Proteobacterium, Acidiphilium cryptum, were brought into pure culture. Bacteria cultured from the stream grow at pH > or =2, and the Cyanidium grows at pH 0.2.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alphaproteobacteria / isolation & purification
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Bacteria / genetics
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Bacteria / isolation & purification*
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Bacteria / ultrastructure
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Betaproteobacteria / isolation & purification
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Chlorobi / isolation & purification
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Evolution, Molecular
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Gene Library
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Gram-Positive Bacteria / isolation & purification
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Hot Temperature*
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Hydrogen-Ion Concentration
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Microscopy, Electron
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New Zealand
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Phylogeny
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 16S / genetics
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Volcanic Eruptions
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Water Microbiology*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S