Expression of COUP-TFII in metabolic tissues during development

Mech Dev. 2002 Nov;119(1):109-14. doi: 10.1016/s0925-4773(02)00286-1.


In mammals, the COUP-TF-family consisting of two structurally related proteins, COUP-TFI and COUP-TFII belongs to the orphan member of the steroid/thyroid hormone receptor superfamily. In an attempt to gain insights into the role of COUP-TFII, we examined developmental expression pattern of the mouse COUP-TFII focusing our studies on endoderm-derived tissues, pancreas and liver in particular. Independent lines of transgenic mice expressing Escherichia coli beta-galactosidase driven by the COUP-TFII promoter were generated. Embryonic expression of the beta-gal protein at day 9 of gestation was detected in the notochord, the ventral neural tube and, interestingly, in the gut endoderm, a site where COUP-TFII has not been detected previously. Between 9.5 and 11.5 dpc, beta-gal expression pattern that was established earlier persisted and sections revealed a staining of the common atrial chamber of the heart. At 15.5 dpc, beta-gal activity was found in all endoderm-derived tissues. We found that COUP-TFII mRNA and protein were present in fetal and adult hepatocytes. Finally, COUP-TFII expression was detected in pancreas, as judged by co-expression of the beta-gal in some of the glucagon and PDX1 positive-cells at 12.5 dpc and co-expression with insulin positive-cells at 15.5 dpc. In adult pancreas, COUP-TFII protein was present in the endocrine islet cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COUP Transcription Factor II
  • COUP Transcription Factors
  • DNA-Binding Proteins / biosynthesis*
  • Endoderm / metabolism
  • Escherichia coli / metabolism
  • Glucagon / metabolism
  • Heart / embryology
  • Hepatocytes / cytology
  • Hepatocytes / metabolism
  • Homeodomain Proteins*
  • Immunohistochemistry
  • Liver / embryology
  • Liver / metabolism
  • Mice
  • Mice, Transgenic
  • Myocardium / metabolism
  • Pancreas / embryology
  • Pancreas / metabolism
  • Plasmids / metabolism
  • RNA, Messenger / metabolism
  • Receptors, Steroid*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Trans-Activators / metabolism
  • Transcription Factors / biosynthesis*
  • beta-Galactosidase / metabolism


  • COUP Transcription Factor II
  • COUP Transcription Factors
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Nr2f2 protein, mouse
  • RNA, Messenger
  • Receptors, Steroid
  • Trans-Activators
  • Transcription Factors
  • pancreatic and duodenal homeobox 1 protein
  • Glucagon
  • beta-Galactosidase