In the immune synapse, ZAP-70 controls T cell polarization and recruitment of signaling proteins but not formation of the synaptic pattern

Immunity. 2002 Oct;17(4):389-99. doi: 10.1016/s1074-7613(02)00421-1.

Abstract

Recognition by T cells of their ligands at the surface of antigen-presenting cells (APCs) leads to T cell activation, polarization of the T cell toward the APC, and formation of an immune synapse. Using ZAP-70-deficient T cells expressing zeta-GFP, we show that ZAP-70 signaling drives the TCR-dependent reorientation of the microtubule-organizing center thus leading to relocation of a zeta-GFP(+) intracellular compartment close to the APC. ZAP-70 is also necessary to supply the synapse with the signaling molecules PKC-theta and LAT. In contrast, ZAP-70 is not required for clustering of zeta-GFP and CD2 or exclusion of CD45 and CD43 from the synapse. These data show that ZAP-70-dependent signaling is required for formation of a functional immune synapse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / physiology
  • Antigens, CD*
  • CD2 Antigens / metabolism
  • Carrier Proteins / metabolism
  • Cell Communication
  • Cell Line
  • Cell Polarity
  • Green Fluorescent Proteins
  • Humans
  • Intercellular Junctions / physiology
  • Isoenzymes / metabolism
  • Jurkat Cells
  • Leukocyte Common Antigens / metabolism
  • Leukosialin
  • Luminescent Proteins / metabolism
  • Lymphocyte Activation
  • Membrane Proteins / metabolism
  • Microtubule-Organizing Center / immunology
  • Microtubule-Organizing Center / physiology
  • Phosphoproteins / metabolism
  • Protein Kinase C / metabolism
  • Protein Kinase C-theta
  • Protein-Tyrosine Kinases / physiology*
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Sialoglycoproteins / metabolism
  • Signal Transduction
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology*
  • ZAP-70 Protein-Tyrosine Kinase

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, CD
  • CD2 Antigens
  • Carrier Proteins
  • Isoenzymes
  • LAT protein, human
  • Leukosialin
  • Luminescent Proteins
  • Membrane Proteins
  • Phosphoproteins
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • Sialoglycoproteins
  • UN1 sialoglycoprotein, human
  • antigen T cell receptor, zeta chain
  • Green Fluorescent Proteins
  • Protein-Tyrosine Kinases
  • ZAP-70 Protein-Tyrosine Kinase
  • ZAP70 protein, human
  • PRKCQ protein, human
  • Protein Kinase C
  • Protein Kinase C-theta
  • Leukocyte Common Antigens