The short N-terminus is required for functional expression of the virus-encoded miniature K(+) channel Kcv

FEBS Lett. 2002 Oct 23;530(1-3):65-9. doi: 10.1016/s0014-5793(02)03397-5.


Kcv (K(+) Chlorella virus) is a miniature virus-encoded K(+) channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Cell Line
  • DNA Primers
  • Green Fluorescent Proteins
  • Humans
  • Luminescent Proteins / genetics
  • Microscopy, Confocal
  • Phycodnaviridae / genetics*
  • Potassium Channels / chemistry
  • Potassium Channels / genetics
  • Potassium Channels / physiology*
  • Recombinant Fusion Proteins / genetics
  • Viral Proteins*


  • DNA Primers
  • Kcv potassium channel, Chlorella virus
  • Luminescent Proteins
  • Potassium Channels
  • Recombinant Fusion Proteins
  • Viral Proteins
  • Green Fluorescent Proteins