Changes of carp FoF1-ATPase in association with temperature acclimation

Am J Physiol Regul Integr Comp Physiol. 2003 Jan;284(1):R153-63. doi: 10.1152/ajpregu.00182.2002. Epub 2002 Sep 12.


Previously we have shown, using two-dimensional electrophoresis, that mitochondrial ATP synthase (F(o)F(1)-ATPase) beta-subunit is the 55-kDa protein increased in cold-acclimated carp Cyprinus carpio (Kikuchi K, Itoi S, and Watabe S. Fisheries Sci 65: 629-636, 1999). To clarify the coordinate expression in various subunits of carp F(o)F(1)-ATPase with temperature acclimation, we examined the differences in mRNA levels of mitochondrial proteins encoded by both nuclear and mitochondrial genes in fast muscle of carp acclimated to 10 and 30 degrees C. The mRNA levels of nuclear genes per unit weight of total RNA were nearly twofold higher in the 10 degrees C- than 30 degrees C-acclimated carp. However, the transcripts of mitochondrial genes for the 10 degrees C-acclimated carp in terms of the same comparing unit were six to seven times as much as those for the 30 degrees C-acclimated carp. The F(o)F(1)-ATPase activities measured at 10, 25, and 30 degrees C were nearly twofold higher for the cold-acclimated fish than their warm-acclimated counterparts. Such quantitative and qualitative changes in carp F(o)F(1)-ATPase may contribute to extra ATP production required to compensate for energy balance at suboptimal temperatures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acclimatization*
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Carps / genetics*
  • Carps / physiology*
  • Cloning, Molecular
  • Cold Temperature*
  • DNA, Complementary / genetics
  • Gene Expression Regulation, Enzymologic*
  • Mitochondrial Proteins / genetics
  • Molecular Sequence Data
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / metabolism
  • Polymerase Chain Reaction
  • Proton-Translocating ATPases / chemistry
  • Proton-Translocating ATPases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Mitochondrial Proteins
  • RNA, Messenger
  • Proton-Translocating ATPases

Associated data

  • GENBANK/AB042438