Sodium and chloride transport by the intestine of the European flounder Platichthys flesus adapted to fresh or sea water

Pflugers Arch. 1975 Jun 26;357(3-4):303-12. doi: 10.1007/BF00585984.

Abstract

1. Everted intestinal sacs prepared from Platichthys flesus adapted to sea water (SW) have higher rates of salt and water transport than did sacs prepared from fresh water adapted (FW) fish. 2. Intestines mounted in Ussing chambers gave stable open-circuit voltages and short-circuit currents after pre-incubation for 20--30 min of --1.91 +/- 0.14 (14) mV and --45.0 +/- 5.1 (14) muA/cm2, SW fish, and --1.24 +/- 0.14 (11) mV and --18.2 +/- 3.6 (11) muA/cm2, FW fish. 3. Isotope flux measurements indicated a net Na transport of 21.5 +/- 4.1 (11) neq/cm2-min in SW fish intestines, but no significant Na transport in FW fish (7.6 +/- 7.6 (9) new/cm2-min). Both preparations showed an active Cl transport, 26.6 +/- 6.1 (12) neq/cm2-min for SW and 18.5 +/- 9.7 (17) neq/cm2-min for FW fish. 4. No significant difference in the uptake of Na or Cl across the mucosa was observed between FW and SW fish. The uptake of both ions showed some saturation at high concentrations. 5. Interactions between Na and Cl uptake were small, although Cl uptake was significantly higher in Na-free solutions. 6. It is concluded that electrogenic Cl transport may be the dominant mechanism for water and salt transport in flounder intestine, and that adaptation to a saline environment involves regulation of pumping rather than Na or Cl entry across the mucosal membrane.

MeSH terms

  • Animals
  • Biological Transport, Active
  • Chlorides / metabolism*
  • Fishes
  • Fresh Water
  • Intestinal Mucosa / metabolism*
  • Intestines / physiology
  • Membrane Potentials
  • Osmolar Concentration
  • Seawater
  • Sodium / metabolism*
  • Water-Electrolyte Balance

Substances

  • Chlorides
  • Sodium