Neoplastic cells and proliferating endothelial cells express connective tissue growth factor (CTGF) in glioblastoma

Neurol Res. 2002 Oct;24(7):677-83. doi: 10.1179/016164102101200573.


Connective tissue growth factor (CTGF) was recently described as a cysteine-rich mitogenic peptide that is the prototypic member of the CCN family of protein including CTGF/fisp12, cef/10/cyr61 and nov. Recently CTGF was reported to have a function of mediating endothelial cell adhesion and migration through integrin alpha(v)beta3, promoting endothelial cell survival, and inducing angiogenesis in vivo. To clarify involvement of CTGF in tumor angiogenesis, using brain tissues with glioblastoma, we examined CTGF expression and localization by immunohistochemical staining and reverse transcription-polymerase chain reaction. The result showed that both glioblastoma tumor cells and proliferating endothelial cells are positive for CTGF by immunohistochemical staining, and CTGF mRNA expression was evaluated by RT-PCR in the brain tissues of glioblastoma. These findings suggest that CTGF may have the potential to stimulate angiogenesis in glioblastoma and function as an angiogenic factor in the process of tumor growth.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Brain Neoplasms / blood supply
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Cell Division / physiology*
  • Child
  • Child, Preschool
  • Connective Tissue Growth Factor
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / pathology
  • Female
  • Glial Fibrillary Acidic Protein / metabolism
  • Glioblastoma / blood supply
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology
  • Humans
  • Immediate-Early Proteins / genetics
  • Immediate-Early Proteins / metabolism*
  • Immunohistochemistry
  • Infant
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Male
  • Middle Aged
  • Neovascularization, Pathologic / metabolism*
  • Neovascularization, Pathologic / physiopathology
  • Nephroblastoma Overexpressed Protein
  • RNA, Messenger / metabolism
  • Up-Regulation / physiology


  • CCN2 protein, human
  • CCN3 protein, human
  • Glial Fibrillary Acidic Protein
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Nephroblastoma Overexpressed Protein
  • RNA, Messenger
  • Connective Tissue Growth Factor