Characterization of a new HLA-DRB3 allele, DRB3*0217, by direct sequencing

Tissue Antigens. 2002 Aug;60(2):189-90. doi: 10.1034/j.1399-0039.2002.600211.x.

Abstract

We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC-->TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Amino Acid Substitution
  • Base Sequence
  • HLA-DR Antigens / classification
  • HLA-DR Antigens / genetics*
  • HLA-DRB3 Chains
  • Humans
  • Male
  • Molecular Sequence Data
  • Point Mutation

Substances

  • HLA-DR Antigens
  • HLA-DRB3 Chains

Associated data

  • GENBANK/AJ441058