Brain-derived neurotrophic factor induces NMDA receptor 1 phosphorylation in rat spinal cord

Neuroreport. 2002 Oct 28;13(15):1967-70. doi: 10.1097/00001756-200210280-00027.

Abstract

The NMDA receptor and the neurotrophin brain-derived neurotrophic factor, BDNF, are involved in central sensitisation and synaptic plasticity in the spinal cord. Recent evidence suggests that BDNF modulates NMDA responses, by a yet unknown mechanism. The phosphorylation of the NMDA subunit 1 following BDNF exposure has been investigated in the neonatal rat spinal cord. Western blotting revealed that BDNF administered to isolated cords for 20 minutes significantly elevated phospho-NR1 levels. Immunohistochemical analysis localised this increase to functionally appropriate regions of the dorsal horn. Pre-incubation with trkB-IgG (BDNF sequestering molecule) abolished this NR1 phosphorylation. We suggest that BDNF modulates synaptic activity within the spinal cord via NR1 phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor / metabolism*
  • Brain-Derived Neurotrophic Factor / pharmacology
  • Immunohistochemistry
  • Neuronal Plasticity / drug effects
  • Neuronal Plasticity / physiology*
  • Neurons / drug effects
  • Neurons / metabolism*
  • Phosphorylation / drug effects
  • Posterior Horn Cells / drug effects
  • Posterior Horn Cells / metabolism
  • Rats
  • Receptors, N-Methyl-D-Aspartate / drug effects
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Spinal Cord / drug effects
  • Spinal Cord / metabolism*
  • Synapses / drug effects
  • Synapses / metabolism*
  • Synaptic Transmission / drug effects
  • Synaptic Transmission / physiology*
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • Brain-Derived Neurotrophic Factor
  • NR1 NMDA receptor
  • Receptors, N-Methyl-D-Aspartate