Immunologic profile of highly exposed yet HIV type 1-seronegative men

AIDS Res Hum Retroviruses. 2002 Sep 20;18(14):1051-65. doi: 10.1089/08892220260235416.


The host immune factors that determine susceptibility to HIV-1 infection are poorly understood. We compared multiple immunologic parameters in three groups of HIV-1-seronegative men: 14 highly exposed (HR10), 7 previously reported possibly to have sustained transient infection (PTI), and a control group of 14 low risk blood bank donors (BB). Virus-specific cellular immune assays were performed for CD4(+) T helper cell responses, CD8(+) cytotoxic T lymphocyte activity, CD8(+) cell chemokine release, and CD8(+) cell-derived antiviral soluble factor activity. General immune parameters evaluated included CCR5 genotype and phenotype, interferon alpha production by PBMCs, leukocyte subset analysis, and detailed T lymphocyte phenotyping. Comparisons revealed no detectable group-specific differences in measures of virus-specific immunity. However, the HR10 group differed from the BB group in several general immune parameters, having higher absolute monocyte counts, higher absolute CD8(+) T cell counts and percentages, lower naive and higher terminal effector CD8(+) cells, and lower levels of CD28(+)CD8(+) cells. These changes were not associated with seropositivity for other chronic viral infections. The PTI men appeared to have normal levels of monocytes and slightly elevated levels of CD8(+) T cells (also with increased effector and decreased naive cells). Although we cannot entirely exclude the contribution of other chronic viral infections, these findings suggest that long-lived systemic cellular antiviral immunity as detected by our assays is not a common mechanism for resistance to infection, and that resistance may be multifactorial. General immune parameters reflected by CD8(+) T cell levels and activation, and monocyte concentrations may affect the risk of infection with HIV-1, and/or serve as markers of exposure.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Chronic Disease
  • Cytokines / biosynthesis
  • HIV Seronegativity / immunology*
  • HIV-1 / immunology*
  • Humans
  • Immunity, Cellular*
  • Immunophenotyping
  • Interferon-gamma / biosynthesis
  • Male
  • Peptides / chemical synthesis
  • Peptides / chemistry
  • Peptides / immunology
  • Receptors, CCR5 / metabolism
  • Retroviridae Proteins / chemistry
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Helper-Inducer / immunology
  • Virus Diseases / immunology


  • Cytokines
  • Peptides
  • Receptors, CCR5
  • Retroviridae Proteins
  • Interferon-gamma